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hek blue htlr reporter cell lines  (InvivoGen)


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    Structured Review

    InvivoGen hek blue htlr reporter cell lines
    Inhibitory effects of isolated compounds on TLR agonist-induced NF-κB/AP-1 activation <t>in</t> <t>HEK-Blue</t> reporter cells. New compounds are shown in green. HEK-Blue cells expressing human (A) TLR2/1, (B) TLR3, (C) TLR4, (D) TLR5, (E) TLR7, or (F) TLR8 were pretreated with MAE (50 μg/mL) or test compounds (50 μM) for 1 h, followed by stimulation with specific agonists: Pam 3 CSK 4 (10 ng/mL), poly­(I:C) (1 μg/mL), LPS (1 μg/mL), FLA-ST (10 ng/mL), imiquimod (1 μg/mL), or R848 (1 μg/mL). After 24 h, SEAP activity, indicating NF-κB/AP-1 pathway activation, was measured at 620 nm. Data are presented as mean ± SEM ( n = 3) from three independent experiments. Significant differences were determined at * p < 0.05, ** p < 0.01, *** p < 0.001 versus vehicle group.
    Hek Blue Htlr Reporter Cell Lines, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek blue htlr reporter cell lines/product/InvivoGen
    Average 96 stars, based on 1240 article reviews
    hek blue htlr reporter cell lines - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "Morutamins A–L from Morus alba L. Root Bark with Inhibitory Effects on Epidermal Growth Factor Receptor and Toll-Like Receptor-Mediated NF-κB/AP‑1 Activation"

    Article Title: Morutamins A–L from Morus alba L. Root Bark with Inhibitory Effects on Epidermal Growth Factor Receptor and Toll-Like Receptor-Mediated NF-κB/AP‑1 Activation

    Journal: ACS Omega

    doi: 10.1021/acsomega.5c11810

    Inhibitory effects of isolated compounds on TLR agonist-induced NF-κB/AP-1 activation in HEK-Blue reporter cells. New compounds are shown in green. HEK-Blue cells expressing human (A) TLR2/1, (B) TLR3, (C) TLR4, (D) TLR5, (E) TLR7, or (F) TLR8 were pretreated with MAE (50 μg/mL) or test compounds (50 μM) for 1 h, followed by stimulation with specific agonists: Pam 3 CSK 4 (10 ng/mL), poly­(I:C) (1 μg/mL), LPS (1 μg/mL), FLA-ST (10 ng/mL), imiquimod (1 μg/mL), or R848 (1 μg/mL). After 24 h, SEAP activity, indicating NF-κB/AP-1 pathway activation, was measured at 620 nm. Data are presented as mean ± SEM ( n = 3) from three independent experiments. Significant differences were determined at * p < 0.05, ** p < 0.01, *** p < 0.001 versus vehicle group.
    Figure Legend Snippet: Inhibitory effects of isolated compounds on TLR agonist-induced NF-κB/AP-1 activation in HEK-Blue reporter cells. New compounds are shown in green. HEK-Blue cells expressing human (A) TLR2/1, (B) TLR3, (C) TLR4, (D) TLR5, (E) TLR7, or (F) TLR8 were pretreated with MAE (50 μg/mL) or test compounds (50 μM) for 1 h, followed by stimulation with specific agonists: Pam 3 CSK 4 (10 ng/mL), poly­(I:C) (1 μg/mL), LPS (1 μg/mL), FLA-ST (10 ng/mL), imiquimod (1 μg/mL), or R848 (1 μg/mL). After 24 h, SEAP activity, indicating NF-κB/AP-1 pathway activation, was measured at 620 nm. Data are presented as mean ± SEM ( n = 3) from three independent experiments. Significant differences were determined at * p < 0.05, ** p < 0.01, *** p < 0.001 versus vehicle group.

    Techniques Used: Isolation, Activation Assay, Expressing, Activity Assay



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    hek blue htlr reporter cell lines  (InvivoGen)
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    InvivoGen hek blue htlr reporter cell lines
    Inhibitory effects of isolated compounds on TLR agonist-induced NF-κB/AP-1 activation <t>in</t> <t>HEK-Blue</t> reporter cells. New compounds are shown in green. HEK-Blue cells expressing human (A) TLR2/1, (B) TLR3, (C) TLR4, (D) TLR5, (E) TLR7, or (F) TLR8 were pretreated with MAE (50 μg/mL) or test compounds (50 μM) for 1 h, followed by stimulation with specific agonists: Pam 3 CSK 4 (10 ng/mL), poly­(I:C) (1 μg/mL), LPS (1 μg/mL), FLA-ST (10 ng/mL), imiquimod (1 μg/mL), or R848 (1 μg/mL). After 24 h, SEAP activity, indicating NF-κB/AP-1 pathway activation, was measured at 620 nm. Data are presented as mean ± SEM ( n = 3) from three independent experiments. Significant differences were determined at * p < 0.05, ** p < 0.01, *** p < 0.001 versus vehicle group.
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    hek blue tm htlr 8 reporter cell lines  (InvivoGen)
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    Impact of the native, partially demannosylated and partially desialylated N -glycans on the inhibition of TLR-8 activation induced with ssRNA40 or R848. HEK293 cells expressing <t>hTLR-8</t> were first incubated with (A) native N -glycans, (B) N -glycans treated with mannosidase, and (C) N -glycans treated with sialidase at 4 mg/mL ( n = 5). After 1 h, the TLR-8 agonists ssRNA40 (50 μg/mL) and R848 (100 μg/mL) were used to stimulate the cells. NF-κB activation was measured by spectrophotometry at 650 nm. Data is represented as mean ± SEM. Statistical differences were measured using paired t -test. Significant differences compared to ssRNA40 and R848 are indicated by * p < 0.05, ** p < 0.01, and **** p < 0.0001.
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    hek blue htlr reporter cell  (InvivoGen)
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    InvivoGen hek blue htlr reporter cell
    Impact of the native, partially demannosylated and partially desialylated N -glycans on the inhibition of TLR-8 activation induced with ssRNA40 or R848. HEK293 cells expressing <t>hTLR-8</t> were first incubated with (A) native N -glycans, (B) N -glycans treated with mannosidase, and (C) N -glycans treated with sialidase at 4 mg/mL ( n = 5). After 1 h, the TLR-8 agonists ssRNA40 (50 μg/mL) and R848 (100 μg/mL) were used to stimulate the cells. NF-κB activation was measured by spectrophotometry at 650 nm. Data is represented as mean ± SEM. Statistical differences were measured using paired t -test. Significant differences compared to ssRNA40 and R848 are indicated by * p < 0.05, ** p < 0.01, and **** p < 0.0001.
    Hek Blue Htlr Reporter Cell, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek blue htlr reporter cell/product/InvivoGen
    Average 96 stars, based on 1 article reviews
    hek blue htlr reporter cell - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Inhibitory effects of isolated compounds on TLR agonist-induced NF-κB/AP-1 activation in HEK-Blue reporter cells. New compounds are shown in green. HEK-Blue cells expressing human (A) TLR2/1, (B) TLR3, (C) TLR4, (D) TLR5, (E) TLR7, or (F) TLR8 were pretreated with MAE (50 μg/mL) or test compounds (50 μM) for 1 h, followed by stimulation with specific agonists: Pam 3 CSK 4 (10 ng/mL), poly­(I:C) (1 μg/mL), LPS (1 μg/mL), FLA-ST (10 ng/mL), imiquimod (1 μg/mL), or R848 (1 μg/mL). After 24 h, SEAP activity, indicating NF-κB/AP-1 pathway activation, was measured at 620 nm. Data are presented as mean ± SEM ( n = 3) from three independent experiments. Significant differences were determined at * p < 0.05, ** p < 0.01, *** p < 0.001 versus vehicle group.

    Journal: ACS Omega

    Article Title: Morutamins A–L from Morus alba L. Root Bark with Inhibitory Effects on Epidermal Growth Factor Receptor and Toll-Like Receptor-Mediated NF-κB/AP‑1 Activation

    doi: 10.1021/acsomega.5c11810

    Figure Lengend Snippet: Inhibitory effects of isolated compounds on TLR agonist-induced NF-κB/AP-1 activation in HEK-Blue reporter cells. New compounds are shown in green. HEK-Blue cells expressing human (A) TLR2/1, (B) TLR3, (C) TLR4, (D) TLR5, (E) TLR7, or (F) TLR8 were pretreated with MAE (50 μg/mL) or test compounds (50 μM) for 1 h, followed by stimulation with specific agonists: Pam 3 CSK 4 (10 ng/mL), poly­(I:C) (1 μg/mL), LPS (1 μg/mL), FLA-ST (10 ng/mL), imiquimod (1 μg/mL), or R848 (1 μg/mL). After 24 h, SEAP activity, indicating NF-κB/AP-1 pathway activation, was measured at 620 nm. Data are presented as mean ± SEM ( n = 3) from three independent experiments. Significant differences were determined at * p < 0.05, ** p < 0.01, *** p < 0.001 versus vehicle group.

    Article Snippet: HEK-Blue hTLR reporter cell lines (InvivoGen) were cultured in 100 mm cell culture dishes with Dulbecco’s Modified Eagle’s Medium (DMEM; HyClone) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Invitrogen), 100 U/mL penicillin-streptomycin (Gibco), and 0.2% Normocin (InvivoGen).

    Techniques: Isolation, Activation Assay, Expressing, Activity Assay

    Impact of the native, partially demannosylated and partially desialylated N -glycans on the inhibition of TLR-8 activation induced with ssRNA40 or R848. HEK293 cells expressing hTLR-8 were first incubated with (A) native N -glycans, (B) N -glycans treated with mannosidase, and (C) N -glycans treated with sialidase at 4 mg/mL ( n = 5). After 1 h, the TLR-8 agonists ssRNA40 (50 μg/mL) and R848 (100 μg/mL) were used to stimulate the cells. NF-κB activation was measured by spectrophotometry at 650 nm. Data is represented as mean ± SEM. Statistical differences were measured using paired t -test. Significant differences compared to ssRNA40 and R848 are indicated by * p < 0.05, ** p < 0.01, and **** p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Inhibitory Effects of Dietary N -Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine

    doi: 10.3389/fimmu.2020.00790

    Figure Lengend Snippet: Impact of the native, partially demannosylated and partially desialylated N -glycans on the inhibition of TLR-8 activation induced with ssRNA40 or R848. HEK293 cells expressing hTLR-8 were first incubated with (A) native N -glycans, (B) N -glycans treated with mannosidase, and (C) N -glycans treated with sialidase at 4 mg/mL ( n = 5). After 1 h, the TLR-8 agonists ssRNA40 (50 μg/mL) and R848 (100 μg/mL) were used to stimulate the cells. NF-κB activation was measured by spectrophotometry at 650 nm. Data is represented as mean ± SEM. Statistical differences were measured using paired t -test. Significant differences compared to ssRNA40 and R848 are indicated by * p < 0.05, ** p < 0.01, and **** p < 0.0001.

    Article Snippet: HEK-Blue TM hTLR-8 reporter cell lines and reagents, such as selection media Quanti-Blue TM reagent, and the agonists single-stranded GU-rich oligonucleotide complexed with LyoVec (ssRNA40/LyoVec TM ) and R848 (resiquimod), were purchased from InvivoGen (Toulouse, France).

    Techniques: Inhibition, Activation Assay, Expressing, Incubation, Spectrophotometry